In Vitro Evaluation of Inhibitory Effect of Artemisinin and Dihydroartemisinin on Calcineurin Enzyme
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چکیده
Materials and Methods: The stable state kinetic for comparison of inhibitory effect of artimisinin, dihydroartimisinin and cyclosporine A on calcineurin activation was used in this study. First, the best inhibitory concentration of artimisinin, dihydroartimisinin and cyclosporine A was calculated. Afterwards, the km and Vmax of them in the presence of substrate, paranitrophenylphosphate (PNPP) were measured. The KI was calculated in the presence of cyclosporine A, artemisinin and dihydroartemisinin. In this study, we used distilled water instead of sample in blank and cyclosporinA as a positive control group.
منابع مشابه
In Vitro Evaluation of Inhibitory Effect of Artemisinin and Dihydroartemisinin on Calcineurin Enzyme
Background and Objective: Artimisinin (an anti-malaria drug) is extracted from Artemisia annua and its water soluble derivative is dihydroartimisinin. Previous in vivo and in vitro studies showed that it has an inhibitory effect on T cells. It is also useful for immunosuppression of immune system. Materials and Methods: The stable state kinetic for comparison of inhibitory effect of artimisinin...
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تاریخ انتشار 2015